Lorene Lanier, Ph.D.

Assistant Professor, Department of Neuroscience

E-MAIL: lanie002@umn.edu

Developmental Plasticity
We are interested in understanding the molecular basis of developmental plasticity.  Current research is focused on medium spiny neurons (MSNs), the major type of neuron in the striatum.  The striatum plays an important role in regulation of movement, motivation and reward and altered or abnormal activity of MSNs is associated with neurological diseases such as Huntington's (HD) and Parkinson's (PD) and is thought to be a major determinant of addiction. These changes in MSN activity have been correlated with changes in dendritic arborization and the number, distribution and morphology of dendritic spines. Current research in the lab uses a recently developed in vitro system are to 1) determine how neurotransmitter signaling affects MSN development and morphology, 2) analyze neurotransmitter receptor trafficking during dendritic spine development and 3) characterize the relationship between MSN development and network excitability. To achieve these goals, we use a combination of multi-electrode array  (MEA) recordings and immunofluorescence microscopy to investigate the relationship between morphometric development and network activity of MSNs. Because it is easily amenable to molecular manipulation and microscopic visualization, future studies may use this in vitro model system to investigate the cellular and molecular mechanisms that regulate MSN development and function, determine the consequences of disease associated gene mutations and screen for drugs that regulate MSN synaptic properties.


Selected Publications:

(For a comprehensive list of recent publications, refer to PubMed, a service provided by the National Library of Medicine.)

Penrod RD, Campagna J, Panneck T, Preese L, Lanier LM. The presence of cortical neurons in striatal-cortical co-cultures alters the effects of dopamine and BDNF on medium spiny neuron dendritic development. Front Cell Neurosci. 2015 Jul 20;9:269.

Penrod RD, Kourrich S, Kearney E, Thomas MJ, Lanier LM. An embryonic culture system for the investigation of striatal medium spiny neuron dendritic spine development and plasticity. J Neurosci Methods. 2011 Aug 30;200(1):1-13. Epub 2011 Jun 13.

Hoover BR, Reed MN, Su J, Penrod RD, Kotilinek LA, Grant MK, Pitstick R, Carlson GA, Lanier LM, Yuan LL, Ashe KH, Liao D. Tau mislocalization to dendritic spines mediates synaptic dysfunction independently of neurodegeneration. Neuron. 2010 Dec 22;68(6):1067-81.

Xu X, Harder J, Flynn DC, Lanier LM. AFAP120 regulates actin organization during neuronal differentiation. Differentiation. 2009;77(1):38-47.PMCID: 2664250

Popko J, Fernandes A, Brites D, Lanier LM. Automated analysis of NeuronJ tracing data. Cytometry A. 2009;75(4):371-6.PMCID: 2661008

Popko J, Fernandes A, Brites D, Lanier LM. Automated analysis of NeuronJ tracing data. Cytometry A. 2009;75(4):371-6.PMCID: 2661008

Fernandes A, Falcao AS, Abranches E, Bekman E, Henrique D, Lanier LM, et al. Bilirubin as a determinant for altered neurogenesis, neuritogenesis, and synaptogenesis. Dev Neurobiol. 2009.PMCID: In Process

Harder J, Xu X, Letourneau P, Lanier LM. The actin cross-linking protein AFAP120 regulates axon elongation in a tyrosine phosphorylation-dependent manner. Neurosci Lett. 2008;444(2):132-6.PMCID: 2575687

Ikin AF, Sabo SL, Lanier LM, Buxbaum JD. A macromolecular complex involving the amyloid precursor protein (APP) and the cytosolic adapter FE65 is a negative regulator of axon branching. Mol Cell Neurosci. 2007;35(1):57-63

Strasser GA, Rahim NA, VanderWaal KE, Gertler FB, Lanier LM. Arp2/3 is a negative regulator of growth cone translocation. Neuron. 2004;43(1):81-94

Former Graduate Students:

Marcela Maldonado (Ph.D. 2009, Neuroscience, University of Minnesota).

Rachel Penrod (Ph.D. 2012, Neuroscience, University of Minnesota).

Lorene Lanier