The overall goal of my laboratory is to minimize or prevent hearing loss by improving our understanding of the structure and function of the normal, impaired, and experimentally altered mammalian inner ear. We are developing the first Mouse Cochlea Database (MCD), which consists of 2D and 3D atlases, and a digital cytocochleogram which is an anatomical map to correlate hair cell damage with frequency-specific, hearing loss. The web page for the Mouse Cochlea Database project can be accessed at the following URL: http://mousecochlea.umn.edu/ We are also interested in the mechanisms of cochlear damage caused by acoustic overstimulation, ototoxic drug administration, Alport's syndrome, and Meniere's disease. The mouse serves as our animal model.
The Cochlear Anatomy Laboratory is located in the Lions Research Building. Our research methods include: immunohistochemistry, thin-sheet, laser optical tomography, confocal, light, transmission, and scanning electron microscopy.
(For a comprehensive list of recent publications, refer to PubMed, a service provided by the National Library of Medicine.)
Johnson SB, Cureoglu S, O'Malley JT, Santi PA. Comparison of traditional histology and TSLIM optical sectioning of human temporal bones. Otol Neurotol. 2014 Aug;35(7):1145-9.
Schmitz HM, Johnson SB, Santi PA. Kanamycin-furosemide ototoxicity in the mouse cochlea: a 3-dimensional analysis. Otolaryngol Head Neck Surg. 2014 Apr;150(4):666-72.
Santi PA, Johnson SB. Decellularized ear tissues as scaffolds for stem cell differentiation. J Assoc Res Otolaryngol. 2013 Feb;14(1):3-15.
Johnson SB, Schmitz HM, Santi PA. TSLIM imaging and a morphometric analysis of the mouse spiral ganglion. Hear Res. 2011 Aug;278(1-2):34-42. Epub 2011 Mar 21.
Santi PA. Light sheet fluorescence microscopy: a review. J Histochem Cytochem. 2011 Feb;59(2):129-38. Review. PMID:21339178[PubMed - indexed for MEDLINE] Related citations
Santi PA, Johnson SB, Hillenbrand M, GrandPre PZ, Glass TJ, Leger JR. Thin-sheet laser imaging microscopy for optical sectioning of thick tissues. Biotechniques. 2009 Apr;46(4):287-94.
Santi PA, Rapson I, Voie A. Development of the mouse cochlea database (MCD). Hear Res. 2008 Sep;243(1-2):11-7. Epub 2008 May 25.
Zehnder AF, Adams JC, Santi PA, Kristiansen AG, Wacharasindhu C, Mann S, Kalluri R, Gregory MC, Kashtan CE, Merchant SN. Distribution of type IV collagen in the cochlea in Alport syndrome. Arch Otolaryngol Head Neck Surg. 2005 Nov;131(11):1007-13.
Santi, P. A., Blair, A., Bohne, B.A., Lukkes, J. and Nietfeld, J. 2004, The Digital Cytocochleogram, Hearing Res., 192: 75-82.
Merchant, S., B.J. Burgess, J.C. Adams, C. E. Kashtan, M.C. Gregory, P. A. Santi , R. Colvin, B. Collins , J. B. Nadol, 2004, Temporal Bone Histopathology in Alport Syndrome, Laryngoscope 11 4: 1609-18.