Stanley A. Thayer, Ph.D.

Professor, Department of Pharmacology

E-MAIL: sathayer@umn.edu

Research Interests:

Dr. Thayer's laboratory studies neurodegenerative processes. His group uses electrophysiological and optical techniques to measure ion currents, to image synaptic proteins and to record changes in intracellular calcium within single neurons grown in tissue culture. Current research efforts focus on three principal areas.

1) Calcium is an essential second messenger involved in neurotransmitter release, gene regulation, and synaptic plasticity. Inappropriate elevation of the intracellular calcium concentration contributes to the neurodegeneration associated with AIDS, ischemia, epilepsy and head trauma. Thus, neurons have developed a complex system to maintain calcium homeostasis. Current studies focus on the modulation of processes that remove calcium from the cytoplasm by drugs and second messengers.

2) Synapse loss occurs early in neurodegenerative disease and correlates with cognitive decline in patients with HIV-1 associated dementia and Alzheimer's disease. Recent studies indicate that loss of synapses is not an early step leading to the cell's demise but instead, is a coping mechanism to reduce excess excitotoxic input. Current studies employ in vitro models to evaluate pharmacological strategies to prevent loss or induce recovery of synapses during neurotoxic processes.

3) Cannabinoids, analogs of the psychoactive ingredient in marijuana, act on receptors that are part of an endogenous signaling system. Endocannabinoid signaling serves as an on-demand neuroprotective mechanism. Currents studies examine the role of the endocannabinoid system in regulating synaptic transmission and neurotoxicity.

Drugs to slow the progression of neurodegenerative disease are lacking. Agents that modulate calcium signals, possibly via the endocannabinoid system, may protect synapses and cells from neurotoxic insult.


Selected Publications:

(For a comprehensive list of recent publications, refer to PubMed, a service provided by the National Library of Medicine.)

Ghosh B, Green MV, Krogh KA, Thayer SA. Interleukin-1β activates a Src family kinase to stimulate the plasma membrane Ca2+ pump in hippocampal neurons. J Neurophysiol. 2016 Feb 3;:jn.00541.2015. [Epub ahead of print]

Krogh KA, Lyddon E, Thayer SA. HIV-1 Tat activates a RhoA signaling pathway to reduce NMDA-evoked calcium responses in hippocampal neurons via an actin-dependent mechanism. J Neurochem. 2015 Feb;132(3):354-66.

Krogh, A.K. Green, M.V. Thayer, S.H. "HIV-1 Tat-induced changes in synaptically-driven network activity adapt during prolonged exposure." 2014;12(6):406-14.

Krogh KA, Wydeven N, Wickman K, Thayer SA. HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca2+ concentration via activation of Src kinase and nitric oxide signaling pathways. J Neurochem. 2014 Sep;130(5):642-56.

Krogh KA, Lyddon E, Thayer SA. HIV-1 Tat activates a RhoA signaling pathway to reduce NMDA-evoked calcium responses in hippocampal neurons via an actin-dependent mechanism. J Neurochem. 2014 Aug 25;.

Feng X, Krogh KA, Wu CY, Lin YW, Tsai HC, Thayer SA, Wei LN. Receptor-interacting protein 140 attenuates endoplasmic reticulum stress in neurons and protects against cell death. Nat Commun. 2014 Jul 28;5:4487.

Krogh KA, Wydeven N, Wickman K, Thayer SA. HIV-1 protein Tat produces biphasic changes in NMDA-evoked increases in intracellular Ca<sup>2+</sup> concentration via activation of Src kinase and nitric oxide signaling pathways. J Neurochem. 2014 Mar 26;.

Shin AH, Kim HJ, Thayer SA. Subtype selective NMDA receptor antagonists induce recovery of synapses lost following exposure to HIV-1 Tat. Br J Pharmacol. 2011 Dec 5. doi: 10.1111/j.1476-5381.2011.01805.x.

Baltgalvis KA, Jaeger MA, Fitzsimons DP, Thayer SA, Lowe DA, Ervasti JM. Transgenic overexpression of γ-cytoplasmic actin protects against eccentric contraction-induced force loss in mdx mice. Skelet Muscle. 2011 Oct 13;1(1):32.

Kim HJ, Shin AH, Thayer SA. Activation of cannabinoid type 2 receptors inhibits HIV-1 envelope glycoprotein gp120-induced synapse loss. Mol Pharmacol. 2011 Sep;80(3):357-66. Epub 2011 Jun 13.

Ghosh B, Li Y, Thayer SA. Inhibition of the plasma membrane Ca2+ pump by CD44 receptor activation of tyrosine kinases increases the action potential afterhyperpolarization in sensory neurons. J Neurosci. 2011 Feb 16;31(7):2361-70.

Roloff AM, Anderson GR, Martemyanov KA, Thayer SA. Homer 1a gates the induction mechanism for endocannabinoid-mediated synaptic plasticity. J Neurosci. 2010 Feb 24;30(8):3072-81.

Kim HJ, Thayer SA. Lithium increases synapse formation between hippocampal neurons by depleting phosphoinositides. Mol Pharmacol. 2009 May;75(5):1021-30. Epub 2009 Feb 2.

Roloff, A. M. and Thayer, S. A. (2009) Modulation of excitatory synaptic transmission by D 9 -tetrahydrocannabinol switches from agonist to antagonist depending on firing rate. Molec. Pharmacol.

Kim, H. J. and Thayer, S. A. (2009) Lithium Increases Synapse Formation between Hippocampal Neurons by Depleting Phosphoinositides. Molec. Pharmacol.

Shideman, C. R., Reinardy J.L. and Thayer, S. A. (2009) g -Secretase Modulates Store Operated Ca 2+ Entry into Rat Sensory Neurons. Neurosci. Lett. 451 :124-8

Kim, H. J., Martemyanov K. A. and Thayer, S. A. (2008) Human Immunodeficiency Virus Protein Tat Induces Synapse Loss via a Reversible Process that is Distinct from Cell Death J. Neurosci . 28 :12604-13.

Kim, H. J., Waataja, J. J and Thayer, S. A. (2008) Cannabinoids inhibit network-driven synapse loss between hippocampal neurons in culture J. Pharmacol. Exp. Ther. 325 : 850-858

Waataja JJ, Kim HJ, Roloff AM, Thayer SA. Excitotoxic loss of post-synaptic sites is distinct temporally and mechanistically from neuronal death. J Neurochem. 2008 Jan;104(2):364-75.


Former Graduate Students:

Matthew Green (Neuroscience, University of Minnesota).

Mariah Wu (Neuroscience, University of Minnesota).

Former Graduate Students:

Jon Waataja (Ph.D. 2007, Neuroscience, University of Minnesota).

Brooke Kelley (Ph.D., Pharmacology, 2004). Postdoctoral Fellow Dept. of Physiology and Biophysics, University of Washington.

Daniel Kim (Ph.D., Pharmacology, 2000). Scientist, R and D Inc, Minneapolis, MN

Guang Jian Wang (Ph.D. 1996, Neuroscience, University of Minnesota). Assistant Professor, Department of Pediatrics, Kosair Children's Hospital Research Institute, University of Louisville

Timothy Piser (Ph.D., Pharmacology, 1995). Director of Neuroscience Research, AstraZeneca Pharmaceuticals, Wilmington, Delaware

John Werth (Ph.D., Pharmacology, 1994). Scientific Liaison, Pfizer Inc., Ann Arbor, Michigan

Stanley Thayer